NYU Langone Medical Center

Sun Lab Reagents

Antibodies to Keratin

 • AE1
 • AE3
 • AE5
 • AE8
 • AE13
 • AE15
 • AE20

Antibodies to Uroplakins

 • AE31
 • AU1.Anti-UPIIIa (pdf)

UPII Promoter

General Information

We have isolated and characterized a 3.6 kb 5'-upstream sequence of mouse uroplakin II gene, and we showed that this sequence can serve as a highly bladder urothelium-specific promoter in transgenic mouse studies. This sequence has been used to drive the mouse bladder-specific expression of human growth factor, SV40-large T antigen, Ha-Ras,  EGF receptor. It has also been used to achieve the urothelium-specific ablation (conventional or inducible) of various genes including Rb and p53. These studies have enabled Dr. Xue-Ru Wu to dissect the roles of various oncogenes and tumore-suppressor genes in bladder tumorigenesis.

This page contains the sequence and restriction map of this mouse UPII promoter (updated from the original paper by Lin et al PNAS 1995). Blasting this sequence against the mouse genome (NCBI) showed 99% identity. While these minor differences may not affect the utility of this promoter in your transgenic experiments, you should keep such differences in mind if you see an unexpected restriction enzyme cut pattern.

This promoter is in a pBluescript SK(+) vector, which contains the Amp-resistant gene. To amplify, transform this into JM109 or DH5 alpha and select on Amp plates and broth.

We recommend the use of KpnI as a 5' restriction enzyme to cut out the promoter. The XhoI and ApaI enzymes do not cut due to a disruption during the subcloning process. Use the map to figure out what enzyme you prefer for the 3' cut. Visit Stratagen for additional information about the vector sequence and restriction map.

If you like to use this promoter for your research or have any questions, please contact Dr.Tung-Tien Sun at sunt01@nyumc.org or Dr. Xue-Ru Wu at Xue-Ru.Wu@nyumc.org.

For more information about the promoter, please view this pdf file: UPII Promoter